BD FACSAriaIII: BMSB J538

The BD FACSAriaIII Flow Cytometer and Cell Sorter is capable of sorting 4 populations simultaneously . The instrument has a digital platform flow cytometer with integrated software (FACSDiVa version 8.0).  Furthermore, it has three lasers:

  • 488nm “Blue” laser detects 5 parameters as well as FSC and SSC.

  • 633nm “Red” laser detects 2 parameters.

  • 405nm “Violet” laser detects 2 parameters.

At 70 psi and 90 kHz with an average threshold rate of 10,000 events per second, a four-way sort achieved a purity of >99% and a yield >80% of expected yield.

 

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