FACS Request

Fluorescence-Activated Cell Sort Request

If you are a member of the CIT and would like to have cells isolated for you using a cell sorter (FACS), please fill out the form below to request a machine and a time to be reserved for you.

Name
Lab*
Email
Sorter to Reserve*
Nozzle
Which nozzle should be inserted into the machine? The rule is that the size of your cells of interest should not exceed one-fifth of the nozzle diameter. Cells that deviate from an ideal spheroid shape are more susceptible to shear induced damage and cell death. If you have a small cell, switching to the larger (85um nozzle) often results in better viability. Nozzle size guideline: 5-10um = 70um nozzle, 11-16um = 85um nozzle & >16um = 100um nozzle.
Active PMTs
Please select the PMT(s) that will need to be active during your sort.
- 405nm -> 450/40 (Pacific Blue)
- 405nm -> 510/50 (Pacific Orange)
- 488nm -> 530/30 (FITC)
- 488nm -> 585/42 (PE)
- 488nm -> 616/23 (PE-Texas Red)
- 488nm -> 695/40 (PerCP-Cy5.5)
- 488nm -> 780/60 (PE-Cy7)
- 633nm -> 660/20 (APC)
- 633nm -> 780/60 (APC-Cy7)
Active PMTs
Please select the PMT that will need to be active during your sort. Fluorochrome choices are listed from high to low intensity.
- FL1 (525/30; VioBright FITC, BB515, Alexa Fluor 488, FITC, DyLight 488, YFP, Fluorescein, GFP, Cy2)
- FL2 (586/25; PE)
- FL3 (615/25; PE-Vio615, PE-CF594, PE-Dazzle594, PE-eFluor610, ECD, DyLight594, PE-Texas Red)
- FL4 (655LP; PE-Vio770, PerCP-eFluor710, PerCP-Vio700, BB700, PE-Cy5.5, PerCP-Cy5.5, PE-Cy7)
Sample Priority*
Please know that we receive rare human samples and may need to reschedule your sort with <24 hours notice.
- (1) nPOD
- (2) Rare Human Clinical Samples
- (3) Human Clinical Samples
- (4) Other Samples
Patient/Sample #
Please let use know if there is a patient or sample ID numbers associated with the samples.
Date*
The sorters are heavily utilized machines. Reservations requests should be made for two weeks or more from today's date.
MM
DD
YYYY
Start Time*
A general guideline is that a sorter can handle 3x10^7 cells per hour.

HH :

MM
End Time*

HH :

MM

When possible, sorts should be begun early enough so that they are completed by 4p.m.

University of Florida

Center for Immunology & Transplantation

Combining Biochemical, Biological, Pharmacological, Molecular, and Clinical Interests.

FACS Request

Fluorescence-Activated Cell Sort Request

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