FACS Request

Which nozzle should be inserted into the machine? The rule is that the size of your cells of interest should not exceed one-fifth of the nozzle diameter. Cells that deviate from an ideal spheroid shape are more susceptible to shear induced damage and cell death. If you have a small cell, switching to the larger (85um nozzle) often results in better viability. Nozzle size guideline: 5-10um = 70um nozzle, 11-16um = 85um nozzle & >16um = 100um nozzle.

Please select the PMT(s) that will need to be active during your sort.

Please select the PMT that will need to be active during your sort. Fluorochrome choices are listed from high to low intensity.

Please let use know if there is a patient or sample ID numbers associated with the samples.